metaboliteProfilingMetadata

Selected Cell

Cell:

Value:

a_metabolome s_BII-S-1 BII-1_investigation Sheet1

100

Source Name

Characteristics[organism]

Term Source REF

Term Accession Number

Characteristics[strain]

Term Source REF

Term Accession Number

Characteristics[genotype]

Term Source REF

Term Accession Number

Protocol REF

Sample Name

Factor Value[limiting nutrient]

Term Source REF

Term Accession Number

Factor Value[rate]

Unit

Term Source REF

Term Accession Number

culture1

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.07-aliquot1

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.07

l/hour

culture1

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.07-aliquot2

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.07

l/hour

culture1

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.07-aliquot3

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.07

l/hour

culture1

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.07-aliquot4

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.07

l/hour

culture1

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.07-aliquot5

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.07

l/hour

culture1

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.07-aliquot6

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.07

l/hour

culture1

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.07-aliquot7

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.07

l/hour

culture1

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.07-aliquot8

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.07

l/hour

culture1

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.07-aliquot9

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.07

l/hour

culture1

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.07-aliquot10

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.07

l/hour

culture2

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.1-aliquot1

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.1

l/hour

culture2

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.1-aliquot2

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.1

l/hour

culture2

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.1-aliquot3

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.1

l/hour

culture2

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.1-aliquot4

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.1

l/hour

culture2

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.1-aliquot5

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.1

l/hour

culture2

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.1-aliquot6

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.1

l/hour

culture2

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.1-aliquot7

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.1

l/hour

culture2

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.1-aliquot8

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.1

l/hour

culture2

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.1-aliquot9

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.1

l/hour

culture2

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.1-aliquot10

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.1

l/hour

culture2

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.1-aliquot11

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.1

l/hour

culture3

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.2-aliquot1

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.2

l/hour

culture3

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.2-aliquot2

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.2

l/hour

culture3

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.2-aliquot3

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.2

l/hour

culture3

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.2-aliquot4

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.2

l/hour

culture3

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.2-aliquot5

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.2

l/hour

culture3

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.2-aliquot6

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.2

l/hour

culture3

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.2-aliquot7

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.2

l/hour

culture3

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.2-aliquot8

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.2

l/hour

culture3

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.2-aliquot9

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.2

l/hour

culture3

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.2-aliquot10

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.2

l/hour

culture3

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

C-0.2-aliquot11

carbon

EFO

http://purl.obolibrary.org/obo/CHEBI_27594

0.2

l/hour

culture4

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.07-aliquot1

nitrogen

0.07

l/hour

culture4

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.07-aliquot2

nitrogen

0.07

l/hour

culture4

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.07-aliquot3

nitrogen

0.07

l/hour

culture4

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.07-aliquot4

nitrogen

0.07

l/hour

culture4

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.07-aliquot5

nitrogen

0.07

l/hour

culture4

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.07-aliquot6

nitrogen

0.07

l/hour

culture4

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.07-aliquot7

nitrogen

0.07

l/hour

culture4

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.07-aliquot8

nitrogen

0.07

l/hour

culture4

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.07-aliquot9

nitrogen

0.07

l/hour

culture4

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.07-aliquot10

nitrogen

0.07

l/hour

culture5

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.1-aliquot1

nitrogen

0.1

l/hour

culture5

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.1-aliquot2

nitrogen

0.1

l/hour

culture5

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.1-aliquot3

nitrogen

0.1

l/hour

culture5

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.1-aliquot4

nitrogen

0.1

l/hour

culture5

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.1-aliquot5

nitrogen

0.1

l/hour

culture5

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.1-aliquot6

nitrogen

0.1

l/hour

culture5

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.1-aliquot7

nitrogen

0.1

l/hour

culture5

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.1-aliquot8

nitrogen

0.1

l/hour

culture5

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.1-aliquot9

nitrogen

0.1

l/hour

culture5

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.1-aliquot10

nitrogen

0.1

l/hour

culture5

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.1-aliquot11

nitrogen

0.1

l/hour

culture6

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.2-aliquot1

nitrogen

0.2

l/hour

culture6

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.2-aliquot2

nitrogen

0.2

l/hour

culture6

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.2-aliquot3

nitrogen

0.2

l/hour

culture6

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.2-aliquot4

nitrogen

0.2

l/hour

culture6

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.2-aliquot5

nitrogen

0.2

l/hour

culture6

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.2-aliquot6

nitrogen

0.2

l/hour

culture6

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.2-aliquot7

nitrogen

0.2

l/hour

culture6

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.2-aliquot8

nitrogen

0.2

l/hour

culture6

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.2-aliquot9

nitrogen

0.2

l/hour

culture6

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.2-aliquot10

nitrogen

0.2

l/hour

culture6

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

N-0.2-aliquot10

nitrogen

0.2

l/hour

culture7

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.07-aliquot1

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.07

l/hour

culture7

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.07-aliquot2

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.07

l/hour

culture7

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.07-aliquot3

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.07

l/hour

culture7

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.07-aliquot4

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.07

l/hour

culture7

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.07-aliquot5

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.07

l/hour

culture7

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.07-aliquot6

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.07

l/hour

culture7

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.07-aliquot7

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.07

l/hour

culture7

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.07-aliquot8

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.07

l/hour

culture7

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.07-aliquot9

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.07

l/hour

culture7

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.07-aliquot10

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.07

l/hour

culture8

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.1-aliquot1

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.1

l/hour

culture8

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.1-aliquot2

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.1

l/hour

culture8

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.1-aliquot3

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.1

l/hour

culture8

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.1-aliquot4

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.1

l/hour

culture8

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.1-aliquot5

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.1

l/hour

culture8

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.1-aliquot6

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.1

l/hour

culture8

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.1-aliquot7

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.1

l/hour

culture8

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.1-aliquot8

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.1

l/hour

culture8

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.1-aliquot9

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.1

l/hour

culture8

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.1-aliquot10

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.1

l/hour

culture8

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.1-aliquot11

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.1

l/hour

culture9

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.2-aliquot1

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.2

l/hour

culture9

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.2-aliquot2

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.2

l/hour

culture9

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.2-aliquot3

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.2

l/hour

culture9

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.2-aliquot4

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.2

l/hour

culture9

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.2-aliquot5

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.2

l/hour

culture9

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.2-aliquot6

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.2

l/hour

culture9

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.2-aliquot7

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.2

l/hour

culture9

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.2-aliquot8

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.2

l/hour

culture9

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.2-aliquot9

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.2

l/hour

culture9

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.2-aliquot10

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.2

l/hour

culture9

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

P-0.2-aliquot11

phosphorus

EFO

http://purl.obolibrary.org/obo/CHEBI_28659

0.2

l/hour

culture10

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

S-0.07-aliquot1

sulfur

0.07

l/hour

culture10

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

S-0.07-aliquot2

sulfur

0.07

l/hour

culture10

Saccharomyces cerevisiae (Baker's yeast)

FY1679

KanMx4 MATa/MATalpha ura3-52/ura3-52 leu2-1/+trp1-63/+his3-D200/+ hoD KanMx4/hoD

growth protocol

S-0.07-aliquot3

sulfur

0.07

l/hour

100

ONTOLOGY SOURCE REFERENCE
Term Source Name	CHEBI	EFO	OBI	EHDAA	PATO	UO
Term Source File	http://data.bioontology.org/ontologies/CHEBI	http://data.bioontology.org/ontologies/EFO	http://data.bioontology.org/ontologies/OBI	http://data.bioontology.org/ontologies/EHDAA	http://data.bioontology.org/ontologies/PATO	http://data.bioontology.org/ontologies/UO
Term Source Version	78	110	21	6	160	42
Term Source Description	Chemical Entities of Biological Interest Ontology	Experimental Factor Ontology	Ontology for Biomedical Investigations	Human Developmental Anatomy Ontology, abstract version 1	Phenotypic Quality Ontology	Units of Measurement Ontology
INVESTIGATION
Investigation Identifier	BII-I-1
Investigation Title	Growth control of the eukaryote cell: a systems biology study in yeast
Investigation Description	Background Cell growth underlies many key cellular and developmental processes, yet a limited number of studies have been carried out on cell-growth regulation. Comprehensive studies at the transcriptional, proteomic and metabolic levels under defined controlled conditions are currently lacking. Results Metabolic control analysis is being exploited in a systems biology study of the eukaryotic cell. Using chemostat culture, we have measured the impact of changes in flux (growth rate) on the transcriptome, proteome, endometabolome and exometabolome of the yeast Saccharomyces cerevisiae. Each functional genomic level shows clear growth-rate-associated trends and discriminates between carbon-sufficient and carbon-limited conditions. Genes consistently and significantly upregulated with increasing growth rate are frequently essential and encode evolutionarily conserved proteins of known function that participate in many protein-protein interactions. In contrast, more unknown, and fewer essential, genes are downregulated with increasing growth rate; their protein products rarely interact with one another. A large proportion of yeast genes under positive growth-rate control share orthologs with other eukaryotes, including humans. Significantly, transcription of genes encoding components of the TOR complex (a major controller of eukaryotic cell growth) is not subject to growth-rate regulation. Moreover, integrative studies reveal the extent and importance of post-transcriptional control, patterns of control of metabolic fluxes at the level of enzyme synthesis, and the relevance of specific enzymatic reactions in the control of metabolic fluxes during cell growth. Conclusion This work constitutes a first comprehensive systems biology study on growth-rate control in the eukaryotic cell. The results have direct implications for advanced studies on cell growth, in vivo regulation of metabolic fluxes for comprehensive metabolic engineering, and for the design of genome-scale systems biology models of the eukaryotic cell.
Investigation Submission Date	2007-04-30T0:0:0+0000
Investigation Public Release Date	2009-03-10T0:0:0+0000
Comment [Created with configuration]
Comment [Last Opened With Configuration]	isaconfig-default_v2013-02-13
Comment [Owning Organisation URI]
Comment [Consortium URI]
Comment [Principal Investigator URI]
Comment [Investigation keywords]
INVESTIGATION PUBLICATIONS
Investigation PubMed ID	17439666	1231222	1234121
Investigation Publication DOI	doi:10.1186/jbiol54
Investigation Publication Author List	Castrillo JI, Zeef LA, Hoyle DC, Zhang N, Hayes A, Gardner DC, Cornell MJ, Petty J, Hakes L, Wardleworth L, Rash B, Brown M, Dunn WB, Broadhurst D, O'Donoghue K, Hester SS, Dunkley TP, Hart SR, Swainston N, Li P, Gaskell SJ, Paton NW, Lilley KS, Kell DB, Oliver SG.	Piatnochka IT.	Monticelli G, Santori S.
Investigation Publication Title	Growth control of the eukaryote cell: a systems biology study in yeast.	Effect of prednisolone on the cardiovascular system in complex treatment of newly detected pulmonary tuberculosis	Indications for the use of prostheses in the treatment of pathological fractures due to primary malignant and metastatic tumours of bone.
Investigation Publication Status	indexed in Pubmed	Published	Published
Investigation Publication Status Term Accession Number
Investigation Publication Status Term Source REF
INVESTIGATION CONTACTS
Investigation Person Last Name	Oliver	Juan	Leo
Investigation Person First Name	Stephen	Castrillo	Zeef
Investigation Person Mid Initials	G	I	A
Investigation Person Email
Investigation Person Phone
Investigation Person Fax
Investigation Person Address	Oxford Road, Manchester M13 9PT, UK	Oxford Road, Manchester M13 9PT, UK	Oxford Road, Manchester M13 9PT, UK
Investigation Person Affiliation	Faculty of Life Sciences, Michael Smith Building, University of Manchester	Faculty of Life Sciences, Michael Smith Building, University of Manchester	Faculty of Life Sciences, Michael Smith Building, University of Manchester
Investigation Person Roles	corresponding author	author	author
Investigation Person Roles Term Accession Number
Investigation Person Roles Term Source REF
Comment[Investigation Person REF]
STUDY
Study Identifier	BII-S-1
Study Title	Study of the impact of changes in flux on the transcriptome, proteome, endometabolome and exometabolome of the yeast Saccharomyces cerevisiae under different nutrient limitations
Study Description	We wished to study the impact of growth rate on the total complement of mRNA molecules, proteins, and metabolites in S. cerevisiae, independent of any nutritional or other physiological effects. To achieve this, we carried out our analyses on yeast grown in steady-state chemostat culture under four different nutrient limitations (glucose, ammonium, phosphate, and sulfate) at three different dilution (that is, growth) rates (D = u = 0.07, 0.1, and 0.2/hour, equivalent to population doubling times (Td) of 10 hours, 7 hours, and 3.5 hours, respectively; u = specific growth rate defined as grams of biomass generated per gram of biomass present per unit time).
Comment[Study Grant Number]
Comment[Study Funding Agency]
Study Submission Date	2007-04-30T0:0:0+0000
Study Public Release Date	2009-03-10T0:0:0+0000
Study File Name	s_BII-S-1.txt
STUDY DESIGN DESCRIPTORS
Study Design Type	intervention design
Study Design Type Term Accession Number	http://purl.obolibrary.org/obo/OBI_0000115
Study Design Type Term Source REF	OBI
STUDY PUBLICATIONS
Study PubMed ID	17439666
Study Publication DOI	doi:10.1186/jbiol54
Study Publication Author List	Castrillo JI, Zeef LA, Hoyle DC, Zhang N, Hayes A, Gardner DC, Cornell MJ, Petty J, Hakes L, Wardleworth L, Rash B, Brown M, Dunn WB, Broadhurst D, O'Donoghue K, Hester SS, Dunkley TP, Hart SR, Swainston N, Li P, Gaskell SJ, Paton NW, Lilley KS, Kell DB, Oliver SG.
Study Publication Title	Growth control of the eukaryote cell: a systems biology study in yeast.
Study Publication Status	published
Study Publication Status Term Accession Number
Study Publication Status Term Source REF
STUDY FACTORS
Study Factor Name	limiting nutrient	rate
Study Factor Type	chemical compound	rate
Study Factor Type Term Accession Number		http://purl.obolibrary.org/obo/PATO_0000161
Study Factor Type Term Source REF		PATO
STUDY ASSAYS
Study Assay File Name	a_metabolome.txt	a_proteome.txt	a_transcriptome.txt
Study Assay Measurement Type	metabolite profiling	protein expression profiling	transcription profiling
Study Assay Measurement Type Term Accession Number	http://purl.obolibrary.org/obo/OBI_0000366	http://purl.obolibrary.org/obo/OBI_0000615	0000424
Study Assay Measurement Type Term Source REF	OBI	OBI	OBI
Study Assay Technology Type	mass spectrometry	mass spectrometry	DNA microarray
Study Assay Technology Type Term Accession Number			http://purl.obolibrary.org/obo/OBI_0400148
Study Assay Technology Type Term Source REF	OBI	OBI	OBI
Study Assay Technology Platform	LC-MS/MS	iTRAQ	Affymetrix
STUDY PROTOCOLS
Study Protocol Name	growth protocol	mRNA extraction	protein extraction	biotin labeling	ITRAQ labeling	EukGE-WS4	metabolite extraction
Study Protocol Type	growth	mRNA extraction	protein extraction	labeling	labeling	hybridization	extraction
Study Protocol Type Term Accession Number							http://purl.obolibrary.org/obo/OBI_0302884
Study Protocol Type Term Source REF							OBI
Study Protocol Description	1. Biomass samples (45 ml) were taken via the sample port of the Applikon fermenters. The cells were pelleted by centrifugation for 5 min at 5000 rpm. The supernatant was removed and the RNA pellet resuspended in the residual medium to form a slurry. This was added in a dropwise manner directly into a 5 ml Teflon flask (B. Braun Biotech, Germany) containing liquid nitrogen and a 7 mm-diameter tungsten carbide ball. After allowing evaporation of the liquid nitrogen the flask was reassembled and the cells disrupted by agitation at 1500 rpm for 2 min in a Microdismembranator U (B. Braun Biotech, Germany) 2. The frozen powder was then dissolved in 1 ml of TriZol reagent (Sigma-Aldrich, UK), vortexed for 1 min, and then kept at room temperature for a further 5min. 3. Chloroform extraction was performed by addition of 0.2 ml chloroform, shaking vigorously or 15 s, then 5min incubation at room temperature. 4. Following centrifugation at 12,000 rpm for 5 min, the RNA (contained in the aqueous phase) was precipitated with 0.5 vol of 2-propanol at room temperature for 15 min. 5. After further centrifugation (12,000 rpm for 10 min at 4 C) the RNA pellet was washed twice with 70 % (v/v) ethanol, briefly air-dried, and redissolved in 0.5 ml diethyl pyrocarbonate (DEPC)-treated water. 6. The single-stranded RNA was precipitated once more by addition of 0.5 ml of LiCl buffer (4 M LiCl, 20 mM Tris-HCl, pH 7.5, 10 mM EDTA), thus removing tRNA and DNA from the sample. 7. After precipitation (20 C for 1h) and centrifugation (12,000 rpm, 30 min, 4 C), the RNA was washed twice in 70 % (v/v) ethanol prior to being dissolved in a minimal volume of DEPC-treated water. 8. Total RNA quality was checked using the RNA 6000 Nano Assay, and analysed on an Agilent 2100 Bioanalyser (Agilent Technologies). RNA was quantified using the Nanodrop ultra low volume spectrophotometer (Nanodrop Technologies).	1. Biomass samples (45 ml) were taken via the sample port of the Applikon fermenters. The cells were pelleted by centrifugation for 5 min at 5000 rpm. The supernatant was removed and the RNA pellet resuspended in the residual medium to form a slurry. This was added in a dropwise manner directly into a 5 ml Teflon flask (B. Braun Biotech, Germany) containing liquid nitrogen and a 7 mm-diameter tungsten carbide ball. After allowing evaporation of the liquid nitrogen the flask was reassembled and the cells disrupted by agitation at 1500 rpm for 2 min in a Microdismembranator U (B. Braun Biotech, Germany) 2. The frozen powder was then dissolved in 1 ml of TriZol reagent (Sigma-Aldrich, UK), vortexed for 1 min, and then kept at room temperature for a further 5min. 3. Chloroform extraction was performed by addition of 0.2 ml chloroform, shaking vigorouslyor 15 s, then 5min incubation at room temperature. 4. Following centrifugation at 12,000 rpm for 5 min, the RNA (contained in the aqueous phase) was precipitated with 0.5 vol of 2-propanol at room temperature for 15 min. 5. After further centrifugation (12,000 rpm for 10 min at 4 C) the RNA pellet was washed twice with 70 % (v/v) ethanol, briefly air-dried, and redissolved in 0.5 ml diethyl pyrocarbonate (DEPC)-treated water. 6. The single-stranded RNA was precipitated once more by addition of 0.5 ml of LiCl bffer (4 M LiCl, 20 mM Tris-HCl, pH 7.5, 10 mM EDTA), thus removing tRNA and DNA from the sample. 7. After precipitation (20 C for 1 h) and centrifugation (12,000 rpm, 30 min, 4 C), the RNA was washed twice in 70 % (v/v) ethanol prior to being dissolved in a minimal volume of DEPC-treated water. 8. Total RNA quality was checked using the RNA 6000 Nano Assay, and analysed on an Agilent 2100 Bioanalyser (Agilent Technologies). RNA was quantified using the Nanodrop ultra low volume spectrophotometer (Nanodrop Technologies).		This was done using Enzo BioArrayTM HighYieldTM RNA transcript labelling kit (T7) with 5 ul cDNA. The resultant cRNA was again purified using the GeneChip ??? Sample Clean Up Module. The column was eluted in the first instance using 10 ???l RNase-free water, and for a second time using 11 ul RNase-free water. cRNA was quantified using the Nanodrop spectrophotometer. A total of 15 ug of cRNA (required for hybridisation) was fragmented. Fragmentation was carried out by using 2 ul of fragmentation buffer for every 8 ul cRNA.		For each target, a hybridisation cocktail was made using the standard array recipe as described in the GeneChip ??? Expression Analysis technical manual. GeneChip ??? control oligonucleotide and 20x eukaryotic hybridisation controls were used. Hybridisation buffer was made as detailed in the GeneChip ??? manual and the BSA and herring sperm DNA was purchased from Invitrogen. The cocktail was heated to 99 C for 5 min, transferred to 45 C for 5 min and then spun for 5 min to remove any insoluble material. Affymetrix Yeast Yg_s98 S. cerevisiae arrays were pre-hybridised with 200 ul 1x hybridisation buffer and incubated at 45 C for 10 min. 200 ul of the hybridisation cocktail was loaded onto the arrays. The probe array was incubated in a rotisserie at 45 C, rotating at 60 rpm. Following hybridisation, for 16 hr, chips were loaded onto a Fluidics station for washing and staining using the EukGe WS2v4 programme controlled using Microarray Suite 5 software.
Study Protocol URI
Study Protocol Version
Study Protocol Parameters Name		test;tes2					sampling
Study Protocol Parameters Name Term Accession Number		;
Study Protocol Parameters Name Term Source REF		;
Study Protocol Components Name
Study Protocol Components Type
Study Protocol Components Type Term Accession Number
Study Protocol Components Type Term Source REF
STUDY CONTACTS
Study Person Last Name	Oliver	Juan	Leo
Study Person First Name	Stephen	Castrillo	Zeef
Study Person Mid Initials	G	I	A
Study Person Email
Study Person Phone
Study Person Fax
Study Person Address	Oxford Road, Manchester M13 9PT, UK	Oxford Road, Manchester M13 9PT, UK	Oxford Road, Manchester M13 9PT, UK
Study Person Affiliation	Faculty of Life Sciences, Michael Smith Building, University of Manchester	Faculty of Life Sciences, Michael Smith Building, University of Manchester	Faculty of Life Sciences, Michael Smith Building, University of Manchester
Study Person Roles	corresponding author	author	author
Study Person Roles Term Accession Number
Study Person Roles Term Source REF