obs unit 1

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first obs unit for seek mife test

SEEK ID: https://sandbox7.fairdomhub.org/observation_units/2340

Extended Metadata (MIFE v1 Observation unit)

Acid flow rate : 1.5 mL/min

Acid type : HCl

Acid weight : 10 g

Aeration rate : 2 vvm

Agitation rate : 200 rpm

Amount of inoculum : 0

Antifoam type : antifoam A

Antifoam weight : 2 g

Bacdive id : 170170

Base flow rate : 1.5 mL/min

Base type : NaOH

Base weight : 10 g

Biosafety level : 1

Compound name : glucose

Concentration : 2 g/L

Concentration method : filtration

Control scheme : temperature

Cultivation type : batch

Culture collection id : DSM 1234

Designations : R7

Dilution rate : 0.05 h-1

Fedbatch rate : 0.8 g/L/h

Fedbatch trigger : glucose depletion

Fedbatch type : constant feeding

Feed flow rate : 0.5 L/h

Feed type : liquid

Feed weight : 100 g

Gas flow rate : 20 L/min

Gas input composition : o2

Gmo : yes

Harvest flow rate : 100 mL/min

Harvest weight : 10 g

Harvesting conditions : 10 degrees celcius

Induction rate : 1 mL/min

Induction trigger : temperature

Induction trigger duration : 50 minutes

Induction type : biological

Initial working volume : 1 L

Inoculation source : preculture flask

Inoculum composition : N/A

Inoculum duration : 100 minutes

Inoculum type : single strain

Input localisation : top feed

Local id : LOC_1

Modifications : life span

Morphology : ring-shaped

Organism : NCBITAXON: 4577

Oxygen dependence : aerobic

Pathogenicity : human

Pco2 : 200 mbar

Phase : log phase

Phase duration : 100 minutes

Phase end time : 2025-07-02T09:57

Phase purpose : growth

Phase start time : 2025-07-01T09:55

Po2 : 500 mbar

Preculture description : not known

Preculture medium : LB

Predox : 250 mV

Protocol : https://ibisibahub.eu/sops/21

Purification method : chromatography

Relative pressure : 2 kPa

Replicate id : R1

Replicate type : biological

Resistance : salt

Resistance mic : 4 mg/L

Sequence accession number : AZ1234

Set temperature : 25 degrees celcius

Source : some lab

Species : maise

Sporulation : no

Substrate flow rate : 01 g/L/h

Target metabolite : ethanol

Taxonomy id : 7556

Temperature of inoculum : 37 degrees celcius

Vessel id : REAC_1

Volume : 100 mL

Water flow rate : 5 mL/min

Weight : 12 g

Internal id : A04

Description : testing obs units 1

Start date : 2025-07-01

Title : obs unit 1

Set pressure : 1 bar

Initial ph : 7.1

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Submitter

Stuart Owen

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Views: 403

Created: 22nd Jul 2025 at 12:35

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